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| Morais,Hévea de; Spuri,Renata; Gonçalves,Tarcísio de Moraes; Carvalho,Rafaela Rodrigues de; Andrade,Renato Campos; Pinto,Tássia Louregiani Carvalho; Souza,José Camisão de. |
| The objective of this study was to investigate whether the use of rbST and eCG prior to ultrasound-guided follicular aspiration (OPU) improves oocyte yield and quality in Tabapuã and Holstein heifers. The study was conducted in two phases, 20 days apart, in a change-over design. The dominant follicle was ablated two days (D-2) before two treatments: stimulation (6 Holstein and 8 Tabapuã), 500 mg of rbST (Boostin®) on D0 and 500 IU of eCG (Folligon) on D2; and control (6 Holstein and 8 Tabapuã), in which heifers received injections of the excipient. Heifers were aspirated on D4. Oocytes were subjected to a well established commercial in vitro embryo production protocol (Vitrogen®) and embryos were evaluated seven days after fertilization. There was an... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Embryo; Hormonal stimulation; Ovary. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200004 |
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| Pinto,Tássia Louregiani Carvalho; Nogueira,Marina Bottrel Reis; Sales,José Nélio de Sousa; Carvalho,Rafaela Rodrigues de; Cushman,Robert Andrew; Souza,José Camisão de. |
| ABSTRACTHigh milk production, heat, physiological status and management impair reproduction in Holstein cows. The use of in vivo-produced embryos has been reported as an alternative to enhance pregnancy outcome in the tropics; however there are several limitations for its production, especially from variations in superovulatory responses. The in vitro production of embryos would avoid such variations, but few studies have been reported. This study aims to verify the effects of variables related to recipients under a program of routine in vitro embryo transfer on a commercial dairy farm in southeastern Brazil. It was hypothesized that pregnancy rates after transfer of ovum pick up or OPUderived embryos (ET) to lactating Holstein recipients may be influenced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine; GnRH agonists; Progesterone. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000500498 |
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| Ruiz López,Salvador; Souza,José Camisão de; Zaraza González,Javier; De Ondiz Sánchez,Aitor; Romero-Aguirregomezcorta,Jon; Carvalho,Rafaela Rodrigues de; Rath,Detlef. |
| The objective was to investigate the effects of sex-sorting on early fertilization events in ovum pick up (OPU) derived oocytes fertilized in vitro with frozen-thawed sperm at different co-incubation lengths. Eighty-four OPU sessions were carried out in 18 cyclic, dry and non-stimulated Holstein Friesian and German black pied cows. Ovum pick up oocytes were matured in vitro for 24 hours and fertilized with frozen-thawed sex sorted or unsorted sperm from the same ejaculate. Fertilization was achieved by two experimental protocols: 1) short gamete co-incubation length: 4, 8 and 12 hours; and 2) long gamete co-incubation length: 18 and 24 hours. After in vitro fertilization, ova were fixed and stained to identify early fertilization events. Sperm penetration,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bovine IVF; Co-incubation length; Early fertilization; Ovum pick up; Sex-sorted sperm. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982013001000005 |
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